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Comparison of lupus patients with early and late onset nephritis: a study in 71 patients from a single referral center
Advances in Rheumatology volume 60, Article number: 5 (2020)
Nephritis occurs frequently in systemic lupus erythematosus (SLE) and may worsen disease morbidity and mortality. Knowing all characteristics of this manifestation helps to a prompt recognition and treatment.
To compare the differences in clinical data, serological profile and treatment response of nephritis of early and late onset.
Retrospective study of 71 SLE patients with biopsy proven nephritis divided in early nephritis group (diagnosis of nephritis in the first 5 years of the disease) and late nephritis (diagnosis of nephritis after 5 years). Epidemiological, serological, clinical and treatment data were collected from charts and compared.
In this sample, 70. 4% had early onset nephritis and 29.6% had late onset. No differences were noted in epidemiological, clinical, serological profile, SLICC and SLEDAI, except that late onset nephritis patients were older at nephritis diagnosis (p = 0.01). Regarding renal biopsy classification, C3 and C4 levels, serum creatinine, 24 h proteinuria and response rate to treatment the two groups were similar (p = NS). Patients with early onset had lower levels of hemoglobin at nephritis onset than those of late onset (p = 0.02).
Most of SLE patients had nephritis in the first 5 years of disease. No major differences were noted when disease profile or treatment outcome of early and late onset nephritis were compared.
Renal involvement in systemic lupus erythematosus (SLE) is one of most common and feared manifestations of this disease as it is related to high morbidity and increased rate of mortality . It has been estimated that almost half of adults and 80–90% of children with systemic lupus will develop kidney involvement  and that 10% of them will go into renal failure [3, 4].
Several factors may affect the prognosis in this context. Ethnic background is one of them; nephritis is more common and more severe in African, Asian and Latin American individuals . Early age at lupus onset and male gender are other factors .
Lupus nephritis is more frequent in those with anti-dsDNA  and it is less common in those with discoid manifestations  and positivity for rheumatoid factor . It usually occurs within the first years after diagnosis  although some patients do develop this complication later on. Few studies [11, 12] address to the characteristics of patients with late onset of nephritis that could allow an early identification and treatment.
Herein we studied systemic lupus patients with nephritis to see if there are differences in clinical, serologic profile and treatment response in patients to analyze those who develop this manifestation early (within the first 5 years) or later in the disease course.
This study was approved by the local Committee of Ethics in Research. It was a retrospective study that included patients with lupus nephritis from a single rheumatology outpatient clinic that attended for regular consultation during the period of 10 years. To be included patients should have SLE diagnosis after 16 years of age, nephritis proved by renal biopsy and received standard treatment for the renal involvement: induction with glucocorticoid, intravenous cyclophosphamide (0.5 to 1.0 g/m2/month for 6 months) or mophetyl mycophenolate (MMF) (2-3g/day - 6 months) and maintenance for at least 2 years with either azathioprine or MMF. Pregnant patients, those who did not complete the treatment and that received any other immunosuppressants were excluded. Clinical and serological data were collected from the charts. The clinical profile was considered in a cumulative manner and collected following the definition of the 1997 American College of Rheumatology revised criteria for the classification of Systemic Lupus Erythematosus ; secondary APS (antiphospholipid antibody syndrome) followed the 2006 modified APS criteria . The autoantibodies tested in the serological profile were: anti-Ro/SS-A, anti-La/SS-B, anti-RNP, anti-Sm, anti-dsDNA, anticardiolipin (aCl) IgG, aCl IgM, LA (lupus anticoagulant), direct Coombs and rheumatoid factor (RF). Anti-Ro/SS-A, anti-La/SS-B, anti-RNP, anti-Sm, aCl-IgG, aCl-IgM were tested by ELISA (using ALKA and Orgentec Kits); anti-dsDNA, by immunofluorescence technique (IFT); the lupus anticoagulant, by screening test, the dRVVT (dilute Russell viper venom test) and confirmed by RVVT. Latex agglutination test (BioSystems) was used to search IgM RF and monoclonal anti human globulin Fresenius-Kabi-Brasil was used for the direct Coombs test.
Data on nephritis included: renal biopsy classification according to ISN/RPS (International Society of Nephrology/Renal Pathology Society) , 24 h proteinuria, creatinine levels, creatinine clearance, values of serum complement fractions C3 and C4, anti-dsDNA positivity and hemoglobin (hb) levels just prior to induction treatment, and after 2 years of treatment. The time from the end of treatment until the first nephritis relapse was also collected.
To be considered as treatment responder patient should have had stabilization or improvement of renal function and reduction of proteinuria to less than 0.5 g / day and /or normal clearence or increase of only up to 10% without active sediment. To be considered as partially responders, they should show reduction of 50% of proteinuria with < 3 g / day and normal clearance or with alteration of up to 10% of the previous value . Non-responders were those with deterioration of renal function after excluding causes such as sepsis, drugs, dehydration and renal vein thrombosis and / or increased proteinuria or non-reduction of proteinuria in order to fall into partial or total remission.
SLEDAI (Systemic Lupus Erythematosus disease activity)  and SLICC/ACR DI (Systemic Lupus International Collaborating Clinics/ American College of Rheumatology Damage Index)  were calculated in the beginning of the treatment and after 2 years.
Patients were divided in two groups, for comparison: (1) those with nephritis that initiated within the first 5 years after SLE diagnosis and classified as early nephritis group; (2) those with nephritis diagnosed more than 5 years after SLE diagnosis and classified as late nephritis group.
Data was collected in frequency and contingency tables. Data distribution was tested by the Shapiro Wilk test. Central tendency was expressed in mean and standard deviation or median and interquartile range (IQR) according to the distribution of studied data. Nominal data were compared by Fisher and chi-squared tests and the numeric, by U-Mann-Whitney and unpaired t tes, respectively. The software Medcalc 10.0 was used for calculations. The adopted significance was of 5%.
Description of studied sample and comparison of clinical and serological data:
Seventy-one patients met the inclusion criteria: 50 (70. 4%) of them had early onset of nephritis and 21 (29.6%) had late onset. Table 1 shows the main characteristics of this group and the comparison between early and late onset nephritis groups.
Comparison of renal involvement in early and late onset nephritis.
The comparison between early and late onset nephritis is on Table 2. In this table it is possible to see that patients from the late nephritis group had a better hemoglobin level in the initial evaluation, showed tendency to recur earlier and had a positive anti-dsDNA more frequently than those in the early nephritis group. Otherwise the two groups had similar results.
We performed a logistic regression - with group of early or late onset as the dependent variable taking into account age, SLICC, SLEDAI and patients age and GNF class. We could not obtain any significance.
Studying only class III and class IV glomerulonephritis, no differences were noted in the remission rate at 1 year (p = 0.72) neither at 2 years (p = 0.30).
Our results showed that almost 2/ 3 of lupus patients developed nephritis in the first 5 years after the diagnosis. This preference for early development of nephritis was already highlighted by Cameron  that emphasized that 25 to 50% of unselected patients with lupus have abnormalities of urine or renal function early in the course of disease. Also, in juvenile lupus up to 80% of patients developing lupus nephritis do it within the first 5 years from diagnosis . The present sample had only adult SLE patients and the age at SLE diagnosis was similar in both groups, but patients in the early nephritis group were younger at nephritis diagnosis. Unfortunately, no other clinical or serological differences could be noted between the two groups that could be associated to the nephritis onset. However our sample was small and may not have had enough strength to demonstrate any differences. It is worthwhile to note that in this sample there were 61.9% of class IV nephritis in the late onset compared to 50% in the early onset group and this data should be take into account to explain the tendency for early relapse in the late onset group. Relapse rate in class IV nephritis is more common .
Regarding treatment, the rate of response was similar in both groups, although a tendency to early relapse was noticed in the late onset nephritis group, with no significance (Table 2). Considering this, patients with late onset nephritis should be treated as aggressively as those of early onset in order to prevent renal damage.
Contrary to our findings, Varela et al.  associated the delayed onset nephritis with the presence of antiphospholipid antibody syndrome (AAF). Our sample had only few cases of AAF that precluded a good observation of this aspect.
This study has some limitations: its retrospective design and the follow up of only 2 years. Another is that nephritis remission was judged only on clinical grounds. It is well known that lupus patients with nephritis may have silent activity only disclosed by repeated biopsy . However, repeated renal biopsy is an aggressive approach not well accepted by all patients. Nevertheless, this study highlights the fact that late and early nephritis have similar outcomes and should not be treated differently.
Our results have shown that nephritis onset is more common in the first 5 years after SLE diagnosis and that lupus patients with early and late onset nephritis share same clinical and serological characteristics. It also shows that these two situations had similar outcomes.
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Clark MR, Trotter K, Chang A. The pathogenesis and therapeutic implications of tubulointerstitial inflammation in human lupus nephritis. Semin Nephrol. 2015;35:455–64.
Brunner HI, Gladman DD, Ibañez D, Urowitz MD, Silverman ED. Difference in disease features between childhood-onset and adult-onset systemic lupus erythematosus. Arthritis Rheum. 2008;58:556–62.
Hanly JG, O’Keeffe AG, Su L, Urowitz MB, Romero-Diaz J, Gordon C, et al. The frequency and outcome of lupus nephritis: results from an international inception cohort study. Rheumatology. 2016;55:252–62.
Ribeiro FM, Fabris CL, Bendet I, Lugon JR. Survival of lupus patients on dialysis: a Brazilian cohort. Rheumatol. 2013;52:494–500.
Isenberg D, Appel GB, Contreras G, Dooley MA, Ginzler EM, Jayne D, et al. Influence of race/ethnicity on response to lupus nephritis treatment: the ALMS study. Rheumatology (Oxford). 2010;49:128–40.
Pons-Estel GJ, Ugarte-Gil MF, Alarcón GS. Epidemiology of systemic lupus erythematosus. Expert Rev Clin Immunol. 2017;13:799–814.
Hsieh SC, Tsai CY, Yu CL. Potential serum and urine biomarkers in patients with lupus nephritis and the unsolved problems. Open Access Rheumatol. 2016;8:81–91 eCollection 2016.
Skare TL, Stadler B, Weingraber E, De Paula DF. Prognosis of patients with systemic lupus erythematosus and discoid lesions. An Bras Dermatol. 2013;88:755–8.
Fedrigo A, Dos Santos TAF, Nisihara R, Skare T. The lupus patient with positive rheumatoid factor. Lupus. 2018;27(8):1368–73.
Ortega LM, Schultz DR, Lenz O, Pardo V, Contreras GN. Lupus nephritis: pathologic features, epidemiology and a guide to therapeutic decisions. Lupus. 2010;19:557–74.
Ugolini-Lopes MR, Santos LPS, Stagnaro C, Seguro LPC, Mosca M, Bonfá E. Late-onset biopsy-proven lupus nephritis without other associated autoimmune diseases: severity and long-term outcome. Lupus. 2019;28:123–8.
Varela DC, Quintana G, Somers EC, Rojas-Villarraga A, Espinosa G, Hincapie ME, et al. Delayed lupus nephritis. Ann Rheum Dis. 2008;67:1044–6.
Hochberg MC. Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythematosus [letter]. Arthritis Rheum. 1997;40:1725.
Miyakis S, Lockshin MD, Atsumi T, Branch DW, Brey RL, Cervera R, et al. International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS). J Thromb Haemost. 2006;4:295–306.
Weening JJ, D'Agati VD, Schwartz MM, Seshan SV, Alpers CE, Appel GB, et al. The classification of glomerulonephritis in systemic lupus erythematosus revisited. Kidney Int. 2004;65:521–30.
Bertsias GK, Tektonidou M, Amoura Z, Aringer M, Bajema I, Berden JH, et al. Joint European league against rheumatism and European renal association-European Dialysis and transplant association (EULAR/ERA-EDTA) recommendations for the management of adult and paediatric lupus nephritis. Ann Rheum Dis. 2012;71:1771–82.
Buyon JP, Petri MA, Kim MY, Kalunian KC, Grossman J, Hahn BH, et al. The effect of combined estrogen and progesterone hormone replacement therapy on disease activity in systemic lupus erythematosus: a randomized trial. Ann Intern Med. 2005;142:953–62.
Gladman D, Ginzler E, Goldsmith C, Fortin P, Liang M, Urowitz M, Bacon P, et al. The development and initial validation of the systemic lupus international collaborating clinics/American College of Rheumatology damage index for systemic lupus erythematosus. Arthritis Rheum. 1996;39:363–9.
Cameron JS. Lupus nephritis. J Am Soc Nephrol. 1999;10:413–24.
Smith EM, Yin P, Jorgensen AL, Beresford MW. Clinical predictors of active LN development in children - evidence from the UK JSLE cohort study. Lupus. 2018;27:2020–8.
Illei GG, Takada K, Parkin D, Austin HA, Crane M, Yarboro CH, et al. Renal flares are common in patients with severe proliferative lupus nephritis treated with pulse immunosuppressive therapy: long-term followup of a cohort of 145 patients participating in randomized controlled studies. Arthritis Rheum. 2002;46:995–1002.
Mok CC, Kwok RC, Yip PS. Effect of renal disease on the standardized mortality ratio and life expectancy of patients with systemic lupus erythematosus. Arthritis Rheum. 2013;65:2154–60.
Houssiau FA, Vasconcelos C, D’Cruz D, Sebastiani GD, de Ramon GE, Danieli MG, et al. Early response to immunosuppressive therapy predicts good renal outcome in lupus nephritis: lessons from long-term follow up of patients in the euro- lupus nephritis trial. Arthritis Rheum. 2004;50:3934–40.
Ishizaki J, Saito K, Nawata M, Mizuno Y, Tokunaga M, Sawamukai N, et al. Low complements and high titer of anti-Sm antibody as predictors of histopathologically proven silent lupus nephritis without abnormal urinalysis in patients with systemic lupus erythematosus. Rheumatology (Oxford). 2015;54:405–12.
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Ethical approval in Committee Ethical Research: 3.155.064.
Consent to participate: consent dismissed by the ethical committee because it was a retrospective study.
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Delfino, J., dos Santos, T.A.F.G. & Skare, T.L. Comparison of lupus patients with early and late onset nephritis: a study in 71 patients from a single referral center. Adv Rheumatol 60, 5 (2020). https://doi.org/10.1186/s42358-019-0105-5
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