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Research | Open | Published:

Mycophenolate mofetil in patients with refractory systemic autoimmune myopathies: case series

Abstract

Background

Currently, there are only few studies (mostly case reports or case series) on mycophenolate mofetil (MMF) in patients with systemic autoimmune myopathies (SAM). Therefore, the goal of the present study was to evaluate the safety and efficacy of MMF (monotherapy or coadjuvant drug) in a specific sample of patients with refractory SAM: dermatomyositis, polymyositis, anti-synthetase syndrome or clinically amyopathic dermatomyositis.

Methods

A case series including 20 consecutive adult patients with refractory SAM from 2010 to 2016 was conducted. After the introduction of MMF, associated or not with other drugs, the patients were followed for 6 consecutive months.

Results

In 17 out of 20 patients MMF was introduced without any intolerance. The clinical symptoms evaluated in these patients were muscular, cutaneous and/or pulmonary activity. During the 6-month follow-up, 11 out of 17 patients had clinical and laboratory activities response with MMF, allowing significant tapering of the prednisone median dose (15 vs. 5 mg/day, P=0.005). On the other hand, in three out of 20 patients; MMF was discontinued in less than two months, because of gastrointestinal intolerance. There were no cases of serious infection or death.

Conclusions

MMF was relatively well-tolerated, safe and effective in patients with refractory SAM. Further studies are needed to confirm the data found.

Background

Systemic autoimmune myopathies (SAM) are a heterogeneous group of rare systemic autoimmune diseases that result in progressive skeletal muscle weakness and disability [1,2,3]. Depending on the demographic, clinical, laboratory, histological and disease evaluation, SAM can be classified into dermatomyositis (DM), polymyositis (PM), inclusion body myositis, or immune-mediated necrotizing myopathy, among others [2,3,4].

There are no randomized controlled clinical trials and glucocorticoid has been used as the first-line drug in SAM [5, 6]. Various immunosuppressive or immunomodulatory drugs have been recommended as glucocorticoid-sparing agents, including methotrexate, azathioprine, cyclosporine, cyclophosphamide, tacrolimus and intravenous human immunoglobulin [5,6,7]. Moreover, the rituximab, an anti-CD20 immunobiological drug, has been administered in refractory SAM cases [7, 8].

Mycophenolate mofetil (MMF) is an agent that inhibits the mitosis and proliferation of T and B lymphocytes and has been successfully used to treat different autoimmune systemic diseases [9]. However, only a few studies in the literature have investigated the use of MMF in adult patients with SAM [10,11,12,13,14,15,16,17,18,19,20]. Furthermore, as limitations, the majority of these studies are case reports or case series [10, 12, 13, 15,16,17,18, 20] and analyzed only SAM patients with pulmonary disease activity [15, 19, 20]. Those who used rituximab [10,11,12,13,14,15,16,17, 19, 20] or anti-synthetase syndrome (ASS) patients [10,11,12,13,14,15,16,17,18,19,20] have not been studied.

The aim of the present case series was to evaluate the safety and efficacy of MMF (monotherapy or coadjuvant drug) in refractory SAM (DM, PM, ASS or clinically amyopathic DM) as monotherapy or in combination of immunosuppressants.

Methods

This retrospective, case series included 21 consecutive adult patients with refractory SAM: classical DM or PM, according to Bohan and Peter’s criteria [21, 22], clinically amyopathic DM, according to Gerami et al. [23], and ASS which was defined as myositis, arthritis, pulmonary disease, positive anti-synthetase antibody, with or without mechanic’s hands, fever and/or Raynaud’s phenomenon [24].

Refractoriness was defined as primarily cutaneous (worsing heliotrope rash and/or Gottron’s sign, new cutaneous lesions attributed to MAS), muscular (objective and progressive limb weakness), articular (arthritis) and/or pulmonary activity (progressive dyspnea), hampering glucocorticoid tapering and/or inadequate response to at least two immunosuppressive or immunomodulatory drugs at full-dose for a minimum period of three months, given sequentially or concomitantly [25].

To improve the homogeneity of the sample under study, only patients followed up at our outpatient clinic between 2010 and 2016 were included.

MMF treatment was defined as effective when the drug promoted over 50% improvement in the initial: cutaneous (evaluated clinically by the rheumatologists from Outgoing clinic); muscular (clinical muscle strength graded according to the Medical Research Council [26]) and/or laboratory parameters (serum creatine phosphokinase level - reference range: 24 - 173 IU/L - assayed by automated kinetic methods)]; articular (arthritis) or pulmonary (subjective dyspnea associated simultaneously with confirmed “ground-glass” on high-resolution chest computed tomography) activity. Comparisons of creatine phosphokinase level values at initial and after 6 months of MMF were considered as expected when variations ranged up to 20%. Moreover, glucocorticoid tapering of over 50% of initial dose was also considered evidence of efficacy of MMF.

All patients were followed for 6 consecutive months after MMF introduction and were examined at baseline and after 6 months by the same examiner.

Myositis overlap syndromes, neoplasia associated myositis, necrotizing myopathies, muscular dystrophy, inclusion body myositis, metabolic myopathies, irregular or doubt treatment adhesions were excluded.

Data were obtained from the ongoing electronic database protocol applied all patients with SAM at 1 - 6 month intervals entailing extensive clinical and laboratory evaluations, including the assessment relevant to this study.

Statistical analysis. The Kolmogorov-Smirnov test was used to evaluate the distribution of each parameter. The demographic and clinical features are expressed as the means ± standard deviations for the continuous variables or as frequencies and percentages for the categorical variables. The medians (25th - 75th percentiles) were calculated for the continuous variables that were not normally distributed. Comparisons between the patients at initial and after 6 months of MMF were performed using Student’s t-test or Wilcoxon test for continuous variables, and P < 0.05 was considered significant. All of the analyses were performed with the SPSS 15.0 statistics software (Chicago, USA).

Results

Twenty consecutive patients with refractory SAM treated with MMF were initially analyzed. In 7 patients, previous immunosuppressive drugs were exchanged for MMF (monotherapy), whereas in 13, MMF was associated with previous immunosuppressant (Table 1).

Table 1 General features of 17 refractory idiopathic systemic autoimmune myopathies

Patients #11 used rituximab 12 months before switch to MMF.

As an internal service protocol, the patients were not using antimalarials, except for one patient (#5).

In 17 out of 20 refractory MAS patients (11 DM, three PM, two ASS, one clinically amyopathic DM) (Table 1), MMF was introduced with good tolerance and with 100% of adhesion. The median dose of MMF was 2 g/day. This group comprised patients that were predominantly women, with a mean age of 46.2 ± 12.6 years and median disease duration of 2.0 years. All 17 patients used glucocorticoids (methylprednisolone or prednisone) and received previously a median of three immunosuppressive drugs (Table 1).

Of this group, 8 had muscle activity, three muscular and skin activities, three cutaneous activities, two pulmonary activities, one cutaneous and pulmonary activity and one had muscular, cutaneous and pulmonary activity. No cases had articular activity.

During the 6-month follow-up, prednisone median dose was significant tapering from 15.0 to 5.0 mg/day (P = 0.005). Moreover, the prednisone tapering was achieved in 14 out of 17 patients. However, glucocorticoid tapering of more than half occurred in 11 patients, all of whom had good clinical activity response using MMF.

As an additional analysis, the MAS patients with MMF as monotherapy (n = 6) were compare to those with MMF in combination therapy (n = 11). All clinical, laboratory, therapeutic and outcome parameters were comparable between both groups (P > 0.05).

In three out of 20 refractory female patients (one DM, one PM and one ASS) with cutaneous, articular and/or muscular activity, MMF was suspended in less than two month, because of gastrointestinal intolerance. The maximum dose of MMF in these patients was 1.5 g/day.

There were no cases of death or infection during the follow-up of the patients analyzed.

Discussion

This case series showed that MMF, as a monotherapy or coadjunt drug, is relatively safe and effective in patients with refractory SAM.

A strict exclusion in rare diseases criteria was employed in this study, however a sample of 20 consecutive patients with refractory SAM was analyzed based on previously standardized and parameterized data. The protocol was performed at the same service adopting the same standardization of reports, thereby reducing inter-examiner variability. Only patients with refractoriness were included.

MMF has been used in several systemic autoimmune diseases, such as systemic sclerosis, rheumatoid arthritis, Sjögre’s syndrome, systemic lupus erythematosus [16, 27,28,29]. However, there are few studies in the literature investigating the use of MMF in adult patients with SAM [10,11,12,13,14,15,16,17,18,19,20].

Most studies are case reports or case series and MMF was found to promote significant clinical and laboratory improvement in patients with SAM [10, 12,13,14,15,16, 19, 20].

According to the study by Majithia and Harisdangkul [10], 6 out of 7 refractory SAM had marked improvement, with good tolerance, in active myositis using MMF. This response rate was higher than ours, however in a group with less severity and in previous use of a smaller number of immunosuppressive drugs.

In another study [13], MMF was effective for controlling cutaneous activity in all four patients with SAM analyzed, also resulting in glucocorticoid tapering. In 10 out of 12 patients with recalcitrant DM, Edge et al. [14] observed an improvement in muscular and cutaneous activity after four weeks of treatment with MMF.

Probably we found a smaller rate of success because of all patients of our sample had refractory and severe disease.

The heterogeneity of response evaluation in myopathies in the literature is present. Better criteria have been established [30,31,32,33], but in relation to DM, for example, there is still a difficulty in assessing improvement, especially in those with little muscle involvement. The response assessment parameter of the present study was based mainly on the clinical criteria.

Previous study showed that antimalarial could predispose patients with DM/PM to developing herpes zoster, particularly women and DM patients [29]. Therefore, as an internal service protocol, our patients were not using antimalarials (except for one patient) at the time of this study.

Facing the previous refractoriness, in two thirds of the patients, the MMF was introduced as a coadjuvant in the present study. However, during follow-up, there was no difference between this group and those who used MMF as monotherapy for the response parameters analyzed.

In the present study, most frequent side effects of MMF were associated with the gastrointestinal tract (nausea, vomiting, abdominal pain and/or diarrhea). Intolerance was observed in three out of the 20 patients in the present analysis, comparable to findings of other studies [12,13,14].

Akin to the present study, some investigations have also shown that MMF is safe in patients with SAM [10, 14, 16]. There were no cases of infection or death events in our sample. By contrast, Rowin et al. [11] reported that three out of their 10 DM patients developed opportunistic infections with MMF (pulmonary infections: Blastomycosis, Mycobacterium xenopi, legionella).

Limitations of this study include the short follow-up of 6 months. In addition, possible inclusion of more severe cases of the disease due to the characteristics of our tertiary care centre should also be considered. Finally, as this is a review of retrospective cases, tools such as Manual Muscle Testing (MMT)-8 [31], 2016 European League Against Rheumatism / American College of Rheumatology (EULAR/ACR) response criteria [34] were not used and pulmonary involvement was not analyzed with pulmonary function test (at baseline and 6 months of MMF) and high-resolution chest computed tomography (6 months of MMF).

Conclusions

MMF was relatively well-tolerated, safe and effective in patients with refractory SAM, at least in the short follow-up of 6 consecutive months. Further studies are needed to confirm the data found in the present study.

Abbreviations

AM:

Antimalarials

ASS:

Anti-synthetase syndrome

AZA:

Azathioprine

C:

Cutaneous

CADM:

Clinically amyopathic dermatomyositis

CP:

Cyclosporine

CPK:

Creatine phosphokinase

CYC:

Cyclophosphamide

DM:

Dermatomyositis

EULAR/ACR:

European League Against Rheumatism / American College of Rheumatology

F:

Female

IVIg:

Intravenous human immunoglobulin

LFN:

Leflunomide

M:

Male

MMF:

Mycophenolate mofetil

MMT:

Manual Muscle Testing

MP:

Methylprednisolone pulse therapy

Mu:

Muscular

P:

Pulmonary

PM:

Polymyositis

Pred:

Prednisone

RTX:

Rituximab

SAM:

Systemic autoimmune myopathies

Tac:

Tacrolymus

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Author information

All authors contributed equally to write and review the manuscript. All authors read and approved the final manuscript.

Correspondence to Samuel Katsuyuki Shinjo.

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This study was approved by the local Ethics Committee (HCFMUSP, CAPPesq, Number 0039/10) and all participant signed the informed consent form.

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Keywords

  • Dermatomyositis
  • Drugs
  • Immunomodulator
  • Immunosuppressive
  • Myositis
  • Polymyositis