Table 2 Summary platelet-rich plasma preparation methods

Ahmad et al.

Double centrifuge method

12 min

300 g

8 min

4000 rpm

N/A

N/A

N/A

10% calcium gluconate

Almasry et al.

Double centrifuge method

20 min

160 g

15 min

400 g

N/A

N/A

0.5 mL of 0.1 M sodium citrate

0.05 mL of 0.2 M calcium chloride

Arican et al.

Double centrifuge method

10 min

96 g

20 min

380 g

1,420,000/μL

1.09 s/μL

Sodium citrate

N/A

Asjid et al.

Double centrifuge method

10 min

500 g

10 min

2200 g

3 times greater than baseline

N/A

0.05 mL of 0.1 M sodium citrate

50 μL of 10% calcium

chloride

Chouhan et al.

Double centrifuge method

20 min

800 rpm

15 min

2200 rpm

≅ 3 times greater than baseline

Low (Unclear)

Acid citrate dextrose

N/A

Coskun et al.

Standard PRP kit

N/A

N/A

N/A

N/A

N/A

N/A

Sodium citrate

Bovine thrombin and calcium chloride

Gamal et al.

Double centrifuge method

8 min

1240 rpm

5 min

1240 rpm

N/A

N/A

Sodium citrate

N/A

Guner & Buyukbebeci

Double centrifuge method

10 min

5600 rpm

10 min

2400 rpm

N/A

N/A

EDTA

N/A

Hermeto et al.

Double centrifuge method

10 min

300 g

10 min

640 g

997.42 ± 48.01/ml

N/A

10% sodium citrate

0.05 mL 10% calcium gluconate

Jayaram et al.

Double centrifuge method

6 min

1500 rpm

15 min

3400 rpm

LP-PRP: 1.556 × 10⁶

± 0.0216 × 10⁶

LR-PRP: 2.760 × 10⁶

± 0.0898 × 10⁶

LP-PRP: 0.970 ± 0.0361

LR-PRP: 3.210 ± 0.6322

100 μL of citrate dextrose solution

N/A

Ji et al.

Unclear

15 min

2000 rpm

-

-

N/A

N/A

0.4 mL of citrate glucose

0.02 mL calcium chloride

Kanwat et al.

Double centrifuge method

20 min

800 rpm

15 min

2200 rpm

G1: 1,820,000/μL

G2: 1,963,000/μL

(≅ 3 times greater than the baseline)

N/A

Acid citrate dextrose

N/A

Kutuk et al.

Standard PRP kit

Unclear

Unclear

Unclear

Unclear

5.24 times greater than the baseline

N/A

2 mL of citrate dextrose

10% calcium chloride

Lu et al.

Double centrifuge method

10 min

1500 g

Unclear

Unclear

N/A

N/A

N/A

N/A

Parlak & Arican

Standard PRP kit

5 min

1700 g

Unclear

Unclear

4N/A8 times greater than the baseline

N/A

3 mL of acid citrate

N/A

Ragab et al.

Double centrifuge method

10 min

1000 rpm

10 min

2000 rpm

N/A

N/A

3.8% sodium citrate

50 μL 10%

calcium chloride

Wang et al.

Standard PRP kit

10 min

350 g

15 min

450 g

1330.1 × 10³/mL

N/A

10 mL acid citrate dextrose

CaCl₂

Wu et al.

Double centrifuge method

10 min

215 g

10 min

863 g

1958.33 ± 316.41 × 10⁹/L

N/A

1 mL of 10% sodium citrate

N/A

Xin et al.

Double centrifuge method

10 min

150 g

10 min

1500 g

1:0 × 10⁹–2:0 × 10⁹/mL

N/A

Acid citrate dextrose

N/A

Yin et al.

Double centrifuge method

10 min

250 g

10 min

1000 g

1947.50 ± 297.71 × 10⁶

56.92 ± 26.83

Acid citrate dextrose

N/A

Yun et al.

Double centrifuge method

10 min

1200 rpm

10 min

2500 rpm

> 1.0 × 10⁶/μL

N/A

7 mL of acid citrate dextrose

N/A