Advances in Rheumatology

Table 2 Primers sequences used for PCR

From: Tetramethylpyrazine alleviates hypoxia-induced proliferation, migration, and inflammatory response of fibroblast-like synoviocytes via inhibiting the HIF-1α- circCDC42BPB pathway

Name		Primers for PCR (5’-3’)
hsa_circ_0004912	Forward	AGCGTGCATTATTTCTGTCTTCTG
hsa_circ_0004912	Reverse	TTACAGTCTTCATTCGGTGCTG
hsa_circ_0005178 circCDC42BPB	Forward	TTACGTGCACAGCTAAACCA
hsa_circ_0005178 circCDC42BPB	Reverse	AACCTCACCAAAAGCACCTCT
hsa_circ_0092297	Forward	GCAGCAGGTGTCTTGCATCT
hsa_circ_0092297	Reverse	TCCCATACAAACAGCCCACTC
hsa_circ_0007514	Forward	CCGGAGGGATTCCATCATAGAG
hsa_circ_0007514	Reverse	GCGGCCGTGCTACAGG
hsa_circ_0047663	Forward	TTGGCAGGTGTGCGCAGAAC
hsa_circ_0047663	Reverse	TAACGTCGGAATGGTACACACTG
CDC42BPB	Forward	GACAAGTACGTGGCCGAGTT
CDC42BPB	Reverse	GCAACCTCACCAAAAGCACCT
HIF-1α	Forward	CGGCGCGAACGACAAGAAAA
HIF-1α	Reverse	GAAGTGGCAACTGATGAGCA
GAPDH	Forward	TCGGAGTCAACGGATTTGGT
GAPDH	Reverse	TTCCCGTTCTCAGCCTTGAC

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ISSN: 2523-3106

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