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Fig. 4 | Advances in Rheumatology

Fig. 4

From: Tetramethylpyrazine alleviates hypoxia-induced proliferation, migration, and inflammatory response of fibroblast-like synoviocytes via inhibiting the HIF-1α- circCDC42BPB pathway

Fig. 4

TMP might inhibit hypoxia-caused RA-FLS proliferation via regulating circCDC42BPB. (A) The overexpression efficiency of circCDC42BPB in RA-FLSs was determined using RT-qPCR (Student’s t-test). (B-E) RA-FLSs were treated with vector, hypoxia + vector, hypoxia + TMP + vector, or hypoxia + TMP + OE-circCDC42BPB. (B) CCK-8 assay was carried out to examine RA-FLS viability (one-way ANOVA). (C) CDC42BPB level was detected using RT-qPCR in RA-FLSs treated with vector, hypoxia + vector, hypoxia + TMP + vector, or hypoxia + TMP + OE-circCDC42BPB (one-way ANOVA). (D) HIF-1α protein level was determined using western blot in RA-FLSs treated with vector, hypoxia + vector, hypoxia + TMP + vector, or hypoxia + TMP + OE-circCDC42BPB (one-way ANOVA). (E) EdU assay was used to examine RA-FLS proliferation (one-way ANOVA). (F) Flow cytometry assay was conducted to measure RA-FLS cycle progression (one-way ANOVA). (G) Western blot analysis of Cyclin D1 and PCNA protein levels (one-way ANOVA). **P < 0.01, ***P < 0.001, n = 3

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Advances in Rheumatology

ISSN: 2523-3106