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Fig. 3 | Advances in Rheumatology

Fig. 3

From: Tetramethylpyrazine alleviates hypoxia-induced proliferation, migration, and inflammatory response of fibroblast-like synoviocytes via inhibiting the HIF-1α- circCDC42BPB pathway

Fig. 3

Expression patterns of circCDC42BPB in TMP-treated RA-FLS under hypoxic conditions. (A) Left, the Venn diagram is the circRNA that is co-upregulated by the GSE226044 (blue) screen in RA synovial tissue and the GSE189338 (yellow) screen in peripheral blood mononuclear cells (PBMCs) from RA patients. Right, the Venn diagram is the circRNA that is co-downregulated by the GSE226044 screen in RA synovial tissue and the GSE189338 screen in PBMCs from RA patients. (B) Expression of these circRNAs was assessed in RA-FLSs treated with hypoxia, hypoxia + TMP, or without by using RT-qPCR (one-way ANOVA). (C) The schematic diagram disclosed the formation of hsa_circ_0005178 (circCDC42BPB) from the CDC42BPB gene. (D) The cellular localization of circCDC42BPB in RA-FLSs was analyzed using Subcellular fractionation assay. (E) CircCDC42BPB and CDC42BPB expression in RA-FLSs with or without RNase treatment was measured using RT-qPCR (one-way ANOVA). (F) RT-qPCR analysis of circCDC42BPB expression in the serum of 25 healthy volunteers and 50 RA patients (Student’s t-test). (G) ROC curve analysis for the diagnostic value evaluation of circCDC42BPB in RA patients. **P < 0.01, ***P < 0.001, n = 3

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Advances in Rheumatology

ISSN: 2523-3106