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Fig. 1 | Advances in Rheumatology

Fig. 1

From: Tetramethylpyrazine alleviates hypoxia-induced proliferation, migration, and inflammatory response of fibroblast-like synoviocytes via inhibiting the HIF-1α- circCDC42BPB pathway

Fig. 1

Effects of TMP on RA-FLS proliferation under hypoxic conditions. RA-FLSs were exposed to hypoxic conditions for 24 h in the presence or absence of TMP (0, 10, 20, and 40 µM). (A) Cell viability was assessed in RA-FLS treated with TMP (0, 10, 20, and 40 µM) using CCK-8 assay (one-way ANOVA). (B and C) Cell viability and proliferation were assessed using CCK-8 and EdU assays (one-way ANOVA). (D) Cell cycle progression was examined using flow cytometry assay (one-way ANOVA). (E) Protein levels of Cyclin D1 and PCNA were measured using western blot assay (one-way ANOVA). *P < 0.05, **P < 0.01, ***P < 0.001, n = 3

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Advances in Rheumatology

ISSN: 2523-3106