Fig. 1
From: TP53 and p21 (CDKN1A) polymorphisms and the risk of systemic lupus erythematosus
Molecular analysis of polymorphisms in the TP53 and p21 (CDKN1A) genes. Left: A TP53 rs1042522 (G > C). Photography of an ethidium bromide-stained agarose gel (2%) showing PCR–BstUI RFLP patterns. Lane 1: 50 bp ladder (INVITROGEN); lanes 2, 3 and 4: genotypes rs1042522 CC (one fragment of 492 or 476 bp), rs1042522 GC (three fragments of 492 or 476, 305 or 284 and 187 bp) and rs1042522 GG (two fragments of 305 or 284 bp and 187 bp), respectively. B TP53 rs17878362 (16 bp Del/Ins). Photography of an ethidium bromide-stained polyacrylamide gel (8%) showing PCR patterns. Lane 1: 50 bp ladder (INVITROGEN); lanes 2, 3 and 4: genotypes rs17878362 A1A1 (one fragment of 492 bp), rs17878362 A1A2 (two fragments of 492 and 476 bp) and rs17878362 A2A2 (one fragment of 476 bp), respectively. C p21 rs1801270 (C > A). Photography of an ethidium bromide-stained polyacrylamide gel (8%) showing PCR–Alw26I RFLP patterns. Lane 1: 50 bp ladder (INVITROGEN); lanes 2, 3 and 4: genotypes rs1801270 CC (two fragments of 105 and 74 bp), rs1801270 CA (three fragments of 179, 105 and 74 bp) and rs1801270 AA (one fragment of 179 bp), respectively. D p21 rs1059234 (C > T) polymorphism. Photography of an ethidium bromide-stained polyacrylamide gel (8%) showing PCR–PstI RFLP patterns. Lane 1: 50 bp ladder (INVITROGEN); lanes 2, 3 and 4: genotypes rs1059234 CC (two fragments of 115 and 68 bp), rs1059234 CT (three fragments of 183, 115 and 68 bp) and rs1059234 TT (one fragment of 183 bp). Right: A–D DNA sequencing—electropherograms corresponding to different genotypes. The polymorphic sites are indicated