Table 6 Cytokine production of non-naive CD4⁺ T-cells from healthy donors (HD) and patients (Pat) with/without MSCs

Cytokine production

% CD4⁺ T-cells

Absolute count

IFNγ-production

TEMRA in %

Absolute count

0.6 ± 0.5 (0.5; 0.1–1.3)

180 ± 150

(150; 30–390)

0.5 ± 0.4 (0.3; 0.1–1.1)

150 ± 120

(90; 30–90)

1.1 ± 1.6 (0.4; 0.1–3.5)

330 ± 480

(180; 30–1050)

0.6 ± 0.6; (0.4; 0.2–1.4)

180 ± 180

(120; 60–420)

Naive in %

Absolute count

0.7 ± 0.5 (0.7; 0.1–1.3)

210 ± 150

(210; 30–390)

0.5 ± 0.4 (0.5; 0.1–1.1)

150 ± 120

(150; 30–330)

2.2 ± 3.9 (0.4; 0.1–8.0)

660 ± 1170

(120; 30–2400)

0.8 ± 1.1 (0.4; 0.2–2.4)

240 ± 330

(120; 60–720)

Memory in %

Absolute count

4.0 ± 3.3 (4.2; 0.1–7.7)

1200 ± 990

(1260; 30–2310)

4.7 ± 1.5 (4.3; 3.5–6.9)

1410 ± 450

(1290; 1050–2070)

4.0 ± 4.2 (3.0; 0.2–9.6)

1200 ± 1260

(900; 60–2880)

5.0 ± 4.7 (3.4; 1.2–11.8)

1500 ± 1410

(1020; 360–3540)

Effector in %

Absolute count

6.0 ± 8.3 (2.3; 0.8–18.4)

1800 ± 2490

(690; 240–5520)

11.2 ± 10.0 (6.8; 5.0–26.1)

3360 ± 3000

(2040; 1500–7830)

3.8 ± 3.0 (3.9; 0.2–7.3)

1140 ± 900

(1170; 60–2190)

9.2 ± 8.3 (7.1; 2.5–20.1)

2760 ± 2490

(2130; 750–6030)

IL-17-production

TEMRA In %

Absolute count

0.1 ± 0.2 (0.0; 0.0–0.3)

30 ± 60

(0; 0–90)

0.1 ± 0.1 (0.0; 0.0–0.2)

30 ± 30

(0; 0–60)

0.9 ± 1.7 (0.1; 0.0–3.5)

270 ± 510

(30; 0–1050)

0.1 ± 0.1 (0.1; 0.0–0.3)

30 ± 30

(30; 0–90)

Naive in %

Absolute count

0.2 ± 0.1 (0.2; 0.0–0.3)

60 ± 30

(60; 0–90)

0.1 ± 0.1 (0.1; 0.0–0.2)

30 ± 30

(30; 0–60)

0.7 ± 1.3 (0.1; 0.0–0.1)

210 ± 390

(30; 0–30)

0.4 ± 0.5 (0.1; 0.1–1.1)

120 ± 150

(30; 30–330)

Memory in %

Absolute count

2.6 ± 2.9 (1.8; 0.3–6.6)

780 ± 870

(540; 90–1980)

1.2 ± 0.7 (1.0; 0.6–2.2)

360 ± 210

(300; 180–660)

0.9 ± 0.6 (0.9; 0.1–1.6)

270 ± 180

(270; 30–480)

1.3 ± 0.9 (1.0; 0.5–2.5)

390 ± 270

(300; 150–750)

Effector in %

Absolute count

3.6 ± 4.1 (2.5; 0.0–9.4)

1080 ± 1230

(750; 0–2820)

6.7 ± 3.9 (7.2; 1.9–10.5)

2010 ± 1170

(2160; 570–3150)

3.9 ± 3.7 (3.4; 0.0–8.9)

1170 ± 1110

(1020; 0–2670)

8.3 ± 5.4 (7.8; 2.9–14.8)

2490 ± 1620

(2340; 870–4440)

IL-9-production

TEMRA in %

Absolute count

0.6 ± 0.5 (0.6; 0.1–1.1)

180 ± 150

(180; 30–330)

0.5 ± 0.4 (0.4; 0.1–1.1)

150 ± 120

(120; 30–330)

1.0 ± 1.4 (0.4; 0.1–3.1)

300 ± 420

(120; 30–930)

0.7 ± 0.5 (0.5; 0.3–1.4)

210 ± 150

(150; 90–420)

Naive in %

Absolute count

0.7 ± 0.5 (0.8; 0.1–1.1)

210 ± 150

(240; 30–330)

0.5 ± 0.4 (0.4; 0.1–1.0)

150 ± 120

(120; 30–300)

1.2 ± 1.3 (0.9; 0.1–3.0)

360 ± 390

(270; 30–900)

0.9 ± 1.0 (0.5; 0.3–2.4)

270 ± 300

(150; 90–720)

Memory in %

Absolute count

1.3 ± 0.8 (0.9; 0.8–2.5)

390 ± 240

(270; 240–750)

3.0 ± 1.6 (2.9; 1.3–4.8)

900 ± 480

(870; 390–1440)

1.1 ± 0.9 (0.8; 0.4–2.5)

330 ± 270

(240; 120–750)

3.2 ± 3.0 (2.1; 1.1–7.6)

960 ± 900

(630; 330–2280)

Effector in %

Absolute count

3.6 ± 5.0 (1.4; 0.4–11.0)

1080 ± 1500

(420; 120–3300)

15.5 ± 14.1 (11.1; 4.4–35.4)

4650 ± 4230

(3330; 1320–10,620)

2.1 ± 2.1 (1.2; 0.7–5.2)

630 ± 630

(360; 210–1560)

14.3 ± 12.5 (12.0; 3.6–29.6)

4290 ± 3750

(3600; 1080–8880)