Table 4 Phenotyping of non-naive CD4⁺ T cells from healthy donors (HD) and patients (Pat) with/without MSCs

TEMRA

CD45RA⁺CD27⁻

%

Absolute count

2.0 ± 1.8 (1.4; 0.6–4.6)

600 ± 540

(420; 180–1380)

2.7 ± 1.5 (2.8; 0.9–4.4)

810 ± 450

(840; 270–1320)

4.7 ± 5.2 (2.7; 1.0–12.2)

1410 ± 1560

(810; 300–3660)

2.5 ± 1.1 (2.3; 1.5–4.0)

750 ± 330

(690; 450–1200)

Naive

CD45RA⁺CD27⁺

%

Absolute count

6.5 ± 2.4 (6.1; 4.2–9.6)

1950 ± 720

(1830; 1260–2880)

9.5 ± 7.5 (8.3; 2.6–19.0)

2850 ± 2250

(2850; 780–5700)

14.3 ± 16.2 (8.3; 2.5–38.2)

4290 ± 4860

(2490; 750–11,460)

6.6 ± 4.8 (7.1; 1.4–11.0)

1980 ± 1440

(2130; 420–3300)

Memory

CD45RA⁺CD27⁺

%

Absolute count

64.4 ± 29.5 (77.4; 20.3–82.6)

19,320 ± 8850

(23,220; 6090–24,780)

35.5 ± 15.9 (40.0; 12.6–49.5)

10,650 ± 4770

(12,000; 3780–14,850)

49.6 ± 28.9 (52.1; 16.3–77.9)

14,880 ± 8670

(14,850; 4890–23,370)

37.1 ± 21.9 (43.3; 6.8–54.9)

11,130 ± 6570

(12,990; 2040–16,470)

Effector

CD45RA⁻CD27⁻

%

Absolute count

27.1 ± 29.3 (13.1; 11.4–71.0)

8130 ± 8790

(3930; 3420–21,300)

52.9 ± 20.6 (46.8; 36.4–81.6)

15,870 ± 6180

(14,040; 10,920–24,480)

31.4 ± 30.6 (17.5; 13.6–77.1)

9420 ± 9180

(5250; 4080–23,130)

53.7 ± 25.4 (47.4; 32.4 – 87.8)

16,110 ± 7620

(16,110; 9720–26,340)