Fig. 4

Triptolide significantly upregulated GL-7⁺B220⁺ cells. A Representative staining of GC B cells(B220⁺GL-7⁺) among mice splenocytes by FACS. B The percentage of GL-7⁺B220⁺ GC B cells (n = 8) in spleen of MRL/lpr mice was significantly higher than that in controlled (C3H) mice (n = 6). The percentage of GL-7⁺B220⁺ GC B cells in spleen of triptolide-treated MRL/lpr mice (n = 10) was significantly lower than that in vehicle-treated MRL/lpr mice (n = 8). C Immunohistochemistry of GCs after triptolide treatment. Cryosection of spleens were stained with PNA (red) and anti-IgD (green) to show GCs in B cell follicles and imaged at 40x magnification. Representative images from four mice per treatment. *p < 0.05, ** p < 0.01