Table 1 Primer sequences of the studied genes in the real-time PCR
Gene name | Primer sequences (5′-3′) |
|---|---|
NOD2 | F: 5′-CACCGTCTGGAATAAGGGTACT-3′ R: 5′- TTCATACTGGCTGACGAAACC-3′ |
P65 | F: 5′-ATGTGGAGATCATTGAGCAGC-3′ R: 5′-CCTGGTCCTGTGTAGCCATT-3′ |
GAPDH | F: 5′-GAGTCAACGGATTTGGTCGT-3′ R: 5′- GACAAGCTTCCCGTTCTCAG-3′ |
IL6 | F: 5′-AATCATCACTGGTCTTTTGGAG-3′ R: 5′-GGTTATTGCATCTAGATTCTTTGC-3′ |
IL1B | F: 5′-ATGGCTTATTACAGTGGCAATGAG3′ R: 5′- GTAGTGGTGGTCGGAGATTCG-3′ |
TNFA | F: 5′-CCTGCCCCAATCCCTTTATT-3′ R: 5′- CCCTAAGCCCCCAATTCTCT-3′ |