Table 1 MSU crystal preparation procedures
Method | Neutralization | Alkali titration | Acid titration | |
|---|---|---|---|---|
Reagent | Uric acid(g) | 0.25 | 4 | 1 |
NaOH(M) | 5 | 0.5 | 1 | |
Volume(mL) | 0.3 | Adjust PH to 8.9 | 6 | |
HCL | - | - | Adjust PH to 7.2 | |
ddH20(mL) | 45 | 800 | 194 | |
Condition | PH | 7.2 | 8.9 | 7.2 |
Temperature | 250℃ | 60℃ | 180℃ | |
Stored | 26℃ for 7 days | 4℃ for 24 h | 4℃ for 24 h | |
Endotoxin removal | 180℃ for 2 h | 180℃ for 2 h | 180℃ for 2 h | |
Endotoxin content | < 0.25EU/mL | < 0.25EU/mL | < 0.25EU/mL | |
Time(day) | 8 | 2 | 2 | |