Fig. 4

The extent of inflammation in the prepared MSU-induced BMDMs. Primary bone marrow mononuclear cells from C57/ BL6 mice were cultured with GM-CSF for 7 days, stimulated for 3 h with LPS (20 ng/mL) and 8 h with MSU crystals (200 µg/mL), and cell supernatants were collected. THP-1 cells were incubated with 50 ng/mL of PMA for 24 h and then stimulated with or without MSU (200 µg/mL) for 8 h. A, C. The levels of IL-1β; B, D. The level of IL-18. IL, interleukin. TNF, tumor necrosis factor. MSU, monosodium urate. BMDMs, bone marrow-derived macrophages. **p＜0.01 for control group compared to other groups; ^$ p＜0.05, ^$$ p＜0.01 for the Alkali titration group compared to other groups