Fig. 3

The degree of inflammation in the prepared MSU -induced air pouch model. 3 mg/1mL MSU suspension was injected into the air pouch, and the lavage fluid harvested at 4 h, 8 h, 12 h (n = 4–6 for each group). A-B, Total cell number was counted by a hematocytometer. C-H, Levels of IL-1β, IL-18 and TNF-α measured by enzyme-linked immunosorbent assays (ELSIA) in air pouch lavage fluid at 4,8, and 12 h. Unpaired t-test was used for each group. IL, interleukin. TNF, tumor necrosis factor. MSU, monosodium urate. ^a p < 0.05, ^A p < 0.01 for different time points of the Neutralization group; ^b p < 0.05, ^B p < 0.01 for different time points of the Alkali titration group; ^c p < 0.05, ^C p < 0.01 for different time points of the Acid titration group; ^d p < 0.05, ^D p < 0.01 for different time points of the Clinical MSU group; ^e p < 0.05, ^E p < 0.01 for different time points of the Commercial MSU group; *p＜0.05, **p＜0.01 for control group compared to other groups; ^#p＜0.05, ^##p＜0.01 for the Neutralization group compared to other groups; ^$ p＜0.05, ^$$ p＜0.01 for the Alkali titration group compared to other groups; ^&p＜0.05, ^&& p＜0.01 for the Acid titration group compared to other groups