Fig. 5

The “crosstalk” between the JAK2 and TGF-β1 signaling pathways in HFLs. A HFLs were treated with TGF-β1 (50 ng/mL) and baricitinib (100 and 1000 nM) or stattic (200 and 400 nM) for 48 h. The protein levels of Col4, SMA, STAT3, p-STAT3, Smad3 and p-Smand3 were analyzed by western blot. B We used 50 nM small RNA interfered STAT3 cultured HFLs. The protein levels of SMA, STAT3, p-STAT3, Smad3 and p-Smand3 were analyzed by western blot. C HFLs were treated with TGF-β1 (50 ng/mL) and LY2109761 (0.5, 1.0 and 2.5 mM) for 48 h. The protein levels of Fn, SMA, JAK2 and TGF-β receptors were analyzed by western blot. Data are presented as the means ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001