Fig. 4

The impacts of TGF-β1, pharmacological inhibition and gene silencing by JAK2 on HFLs. A HFLs were treated with different concentrations of TGF-β1 (0, 10, 50, 100 and 200 ng/mL) for 48 h. The protein levels of Fn, Col4, SMA, JAK2 and TGF-β receptors were analyzed by western blot. B HFLs were treated with TGF-β1 (50 ng/mL) and baricitinib (10,100 and 1000 nM) for 48 h. The protein levels of Fn, SMA, JAK2 and TGF-β receptors were analyzed by western blot. C We used TGF-β1 (50 ng/mL) and 50 nM small RNA interfered JAK2 cultured HFLs. The protein levels of SMA, JAK2 and TGF-β receptors were analyzed by western blot. Data are presented as the means ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001