Fig. 3
From: SIRT1 is transcriptionally repressed by YY1 and suppresses ferroptosis in rheumatoid arthritis
Overexpression of SIRT1 inhibited LPS-induced ferroptosis of synovial cells. Synoviocytes were transfected with pcDNA-3.1 or pcDNA-SIRT1. A The mRNA expression of SIRT1 was examined by qPCR. B The protein level of SIRT1 was examined by western blot. C Cell viability was assessed by CCK-8 assay. D ROS level was detected by DCFH-DA fluorescent probe. E Iron level in the synoviocytes was examined by iron assay kit. Experiments were carried out in triplicate, mean ± SD, **p < 0.01; ***p < 0.001