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Fig. 3 | Advances in Rheumatology

Fig. 3

From: PLCL1 regulates fibroblast-like synoviocytes inflammation via NLRP3 inflammasomes in rheumatoid arthritis

Fig. 3

Effect of PLCL1-siRNA inhibited FLS Expressing inflammatory cytokines. a, b Detecting transfection efficiency of the three transfected strands in RA FLS by RT-QPCR and WB. c The level of PLCL1 protein in 10 ng/ml TNF-α-incubated RA FLS were analyzed by WB, after using PLCL1siRNA. d The mRNA level of IL-6, CXCL8 and IL-1β was analyzed by RT-QPCR in TNF-α-incubated in RA FLS after using PLCL1siRNA. e ELISA was used to detect the production of IL-6, CXCL8 and IL-1β in TNF-α-incubated RA FLS supernatants after using PLCL1-siRNA. f The protein level of IL-6 and IL-1β was analyzed by WB in TNF-α-incubated in RA FLS after using PLCL1-siRNA. (Compared with the RA group # P < 0.05, ## P < 0.01; compared with the negative control group * P < 0.05, ** P < 0.01.)

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Advances in Rheumatology

ISSN: 2523-3106