Fig. 2

Effect of stimulating RA FLS imitating the inflammatory microenvironment in joint cavity of RA patients. a The mRNA level of PLCL1 in RA FLS was analyzed by RT-QPCR after being stimulated by IL-17A, IFN-γ, CXCL8, TNF-a, IL-1β, IL-6 and LPS respectively. b The protein level of PLCL1 in RA FLS was analyzed by WB after being stimulated by 0, 2.5, 5, 10 and 20 ng/ml TNF-α. c The mRNA level of IL-6, CXCL8 and IL-1β in RA FLS was analyzed by RT-QPCR after being stimulated by 0, 2.5, 5, 10 and 20 ng/ml TNF-α. d The mRNA level of MMP-3 and MMP-9 was analyzed by RT-QPCR in RA FLS after being stimulated by 0, 2.5, 5, 10 and 20 ng/ml TNF-α. e The expression of PLCL1 and Vimentin were analyzed by double immunofuorescence staining analysis in RA FLS before and after being stimulated by 10 ng/ml TNF-α. (Compared with the RA group ^# P < 0.05, ^## P < 0.01.)