Fig. 5

Whole body autoradiography in mCIA model. Comparison of mCIA mice dosed with ¹²⁵I labeled anti-FnEDA (left) and control ¹²⁵I labeled anti-TeTx mAb (right) (A). IHC detection of anti-FnEDA mAbs overlaid with the radio signal (B). Representative paws from animals dosed with Anti-FnEDA or Anti-TeTx (C). For each paw, 3 circles with identical area were drawn over high intensity areas (D), and the radioactive signal from these circles were converted to amount of antibody based on a standard curve and plotted (E). (Red bar represents the average amount of antibody extrapolated from such areas in joints of mice treated with anti-FnEDA mAb with standard deviation, N = 9. Blue bar represents the average amount of antibody extrapolated from such areas in joints of mice treated with anti-TeTx mAb with standard deviation, N = 9.)