Fig. 4

TRPS1 was targeted by miR-126-5p. a The binding sequences between TRPS1 and miR-126-5p were predicted by starbase. b The interaction between TRPS1 and miR-126-5p in CHON-001 cells was verified by dual-luciferase reporter (DLR) assay. ^**P < 0.01, vs. mimics NC. c Relative protein expression of TRPS1 was detected by western blot in CHON-001 cells. ^***P < 0.001, vs. mimics NC. ^###P < 0.001, vs. inhibitor NC. d Relative expression of TRPS1 was detected by qRT-PCR in cartilage tissues of PTOA patients and normal cartilage tissues. ^***P < 0.001, vs. Control. e The correlation between TRPS1 and miR-126-5p was analyzed by Pearson’s correlation analysis in cartilage tissues of PTOA patients. P = 0.0037. R² = 0.336. f The correlation between KCNQ1OT1 and TRPS1 was analyzed by Pearson’s correlation analysis in cartilage tissues of PTOA patients. P < 0.0001. R² = 0.6136