Fig. 2
From: SIRT1 is transcriptionally repressed by YY1 and suppresses ferroptosis in rheumatoid arthritis
SIRT1 was downregulated while ferroptosis and YY1 was upregulated in LPS-induced synoviocytes. A CCK-8 assay assessed viability. B ROS production was detected by DCFH-DA fluorescent probe. C Iron level in the synoviocytes was examined by iron assay kit. D The mRNA expressions of YY1 and SIRT1 in the synoviocytes were examined by qPCR. E The protein levels of YY1 and SIRT1 were detected by western blot. Experiments were carried out in triplicate, mean ± SD, **p < 0.01; ***p < 0.001