Fig. 5

Effect of TNF-α and IL-1β on the activation of the MAP kinase (p-ERK1/2, p-P38, and p-JNK) pathway and the transcription factor NF-κB in Fibroblast-like synoviocytes from patients with rheumatoid arthritis (RA-FLSs) and mice with collagen-induced arthritis (CIA-FLSs). RA-FLS and CIA-FLS were stimulated for 30 min with TNF-α and IL-1β. β-actin and phosphorylated proteins p38, ERK1/2, JNK and NF-κB expression levels were examined by western blotting. A–D The phosphorylated proteins ERK1/2, p38, JNK and NF-κB in RA-FLS. E–G The phosphorylated proteins ERK1/2, p38 and NF-κB in CIA-FLS. H Representative western blot figure. (Kruskal–Wallis one-way test, p < 0.05) NS = unstimulated, TNF-α = tumor necrosis factor alpha, IL-1 beta = interleukin 1 beta