Fig. 3

F. hepatica extract did not alter parameters of nuclear morphometry, apoptosis, cell death of FLS. A Nuclear morphometric analysis was performed in FLS cell after 48 h culture with F. hepatica extract (100 mg/mL). Representative analysis in parameters of area, roundness, aspect, radius ratio, area box, and the nuclear irregularity index NII in parameters of nuclear irregularity when compared with the untreated group. Red dots: control; blue dots: F. hepatica extract; (n = 3) B Percentage of positive cells for viability parameters (n = 3). C Representative flow cytometry analysis of apoptosis (Annexin V-FITC, BL1 axis) and necrosis (propidium iodide, BL3 axis); Control (left) and treated (right). Nonstained control is also showed. Q1 (Annexin-/PI-): viable cells; Q2 (Annexin + /PI-): early apoptotic cells; Q3 (Annexin-/PI +): necrotic cells; Q4 (Annexin + /PI +): late apoptotic cells; D Percentage of viable, apoptotic, and dead cells (n = 4). Data are expressed as mean ± SD