Fig. 5

PLCL1 plays an important function under the regulation of the NLRP3 inflammasomes. a The level of NLRP3 protein and Caspase-1/Cleaved Caspase-1 protein in 10 ng/ml TNF-α-incubated RA FLS were analyzed by WB after using PLCL1siRNA. b The protein level of NLRP3 and Caspase-1/Cleaved Caspase-1 were analyzed by WB in 10 ng/ml TNF-α-incubated RA FLS after using PLCL1 overexpression plasmids. c WB was used to detect NLRP3, Caspase-1/Cleaved Caspase-1, IL-6 and IL-1β protein levels in 10 ng/ml TNF-α-incubated RA FLS after using PLCL1 overexpression plasmids and NLRP3 inhibitor INF39. (Compared with the RA group ^# P < 0.05, ^## P < 0.01; compared with the negative control group ^* P < 0.05, ^** P < 0.01; compared with the PLCL1 overexpression group ^& P < 0.05, ^&& P < 0.01.)