Fig. 4

Effect of PLCL1 overexpression promoted FLS expressing inflammatory cytokines. a The overexpression efficiency of PLCL1 were analyzed by RT-QPCR in RA FLS. b The protein levels of PLCL1 were analyzed by WB in TNF-α-incubated in RA FLS after using PLCL1 overexpression plasmids. c The mRNA levels of IL-6, CXCL8 and IL-1β were analyzed by RT-QPCR in TNF-α-incubated in RA FLS after using PLCL1 overexpression plasmids. d ELISA was used to detect the production of IL-6, CXCL8 and IL-1β in TNF-α-incubated RA FLS supernatants after using PLCL1 overexpression plasmids. e The protein levels of IL-6 and IL-1β were analyzed by WB in 10 ng/ml TNF-α-incubated in RA FLS after using PLCL1 overexpression plasmids. (Compared with the RA group ^# P < 0.05, ^## P < 0.01; compared with the negative control group ^* P < 0.05, ^** P < 0.01.)